ZeroBkg® Surface Coating Products

We provide functionalized surfaces for the immobilization of proteins, antibobies, peptides, carbohydrates, and other biomolecules. All products are made from a proprietary coating which consists of a high density poly-ethylene-glycol (PEG) brush. It offers exceptionally low background and an ideal environment for biomolecule adsorption. The surface of the PEG brush is further functionalized to meet the particular need of immobilization. The immobilized probe has little interaction with the surface besides the linker, thus maintaining optimal activity. The density of functinal groups on a surface can also be controlled to accomodate different applications, such as single molecule spectroscopy, atomic force microscopy, protein microarrays, microfluidics, etc. These coatings are supplied on glass slides, coverslips, silicon wafers, ITO, and other customer materials.

Product types	Applications
1. PEG brush as a zero background surface	A zero background surface is ideal for biophysical experiments, such as single molecule spectroscopy, biological atomic force microscopy, and as starting surfaces for bioconjugation .
2. Biotin/streptavidin surface	This product comes either in the form of a biotin surface for activation with streptavidin or in the form of pre-adsorbed streptavidin. The surface is used for the selective immobilization of biotin-labeled molecules. The PEG environment ensures exceptionally low background. Control streptavidin amount at the surface is important (see application note).
3. Chelated Cu surface	This is an ideal surface for the immobilization of poly-histidine tagged protein or peptides. A protein molecule is linked to the surface only through the poly-His tag with controlled orientation but otherwise stays away from the surface as much as possible. This ensures protein activity of the native state. No sample pre-purification is needed.
4. NHS surfaces	The advantage of this surface is that it allows for very fast immobilization and the remaining chemical groups are easily washed off to expose the zero background PEG brush. Ideal for ultra-small quantities and crude tissue samples.
5. Maleimide surfaces	Covalent linking between a maleimide group and a sulfhydryl group is one of the most selective, facile, and convenient reactions in bioconjugation chemistry. We have now made this favorite reaction available on the ZeroBkg® surface.
6. Alkyne surfaces	The so-called "Click chemistry" is becoming one of the most reliable conjugation strategies in organic and biochemistry. One can easily link azide (-N3) functionalized molecules to the alkyne groups on the ZeroBkg® surface. The reaction is extremely efficient, selective, and facile.
7. Fluidic and air-stable cell membrane	The latest ground breaking technology from MicroSurfaces: supported lipid bilayer, which remains fluidic after repeated exposure to air, drying, and rehydration. This allows easy fabrication of a variety of cell membrane mimicking microarrays from simple robotic spotting/arraying.

3913 Todd Lane, Suite 102, Austin, TX 78744
Phone: (512) 916-0802; Fax: (512) 916-0855; E-mail: